HSF1 antibody (C-Term)

Catalog No. ABIN190697

The Rabbit Polyclonal anti-HSF1 antibody has been validated for WB. It is suitable to detect HSF1 in samples from Chlamydomonas reinhardtii.

Quick Overview for HSF1 antibody (C-Term) (ABIN190697)

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Reactivity: Chlamydomonas reinhardtii

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HSF1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-HSF1 Antibody

Binding Specificity: C-Term

Cross-Reactivity (Details): Not reactive in: higher plants

Predicted Reactivity: Chlamydomonas reinhardtii

Characteristics: Expected / apparent Molecular Weight of the Antigene: 78.4 / 100 kDa

Purification: serum

Immunogen: C-terminal part of Chlamydomonas reinhardtii HSF1 (Exons 7 to 9), UniProt: A1YTN3

Application Details

Application Notes: 1. 3000 with standard ECL (WB)

Comment:

unspecific cross-reactions at 40-50 kDa

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: For reconstitution add 100 μL of sterile water.

Handling Advice: Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles.

Storage: -20 °C

Storage Comment: store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.

Target Details for HSF1

Target: HSF1 (Heat Shock Factor Protein 1 (HSF1))

Alternative Name: HSF1

Background: HSF1 is a key regulator of the stress response. It becomes hyperphosphorylated following heat shock (results in the shifting of the HSF1 protein to slightly higher MW) and HSF1 protein rapidly accumulates during heat stress. HSF1 appears to be constitutively present as high molecular weight complexes, presumably trimers. It also interacts with cytosolic HSP70A.

Molecular Weight: expected: 78.4 kDa, apparent: 100 kDa

UniProt: A1YTN3